Testing for maternal contamination

Test covered by the reimbursement:

YES

Gender:

Woman

Material:

Peripheral blood, Isolated DNA from blood

STATIM:

2 days

Material:

Peripheral blood | 1x 3 ml of whole blood in K3 EDTA tube

Storage after examination: week after the report is issued 2 – 8°C

Isolated DNA from blood | 10–100 ng/μL of isolated DNA from blood in a PCR tube of at least 15 μL.

Storage after examination: stored in a DNA archive without restriction 15°C

Quick test description:

Testing of maternal contamination of material after collection by invasive techniques, using QF-PCR.

Test details:

The testing serves as a quick confirmation or exclusion of maternal contamination in the amniotic fluid sample. It is performed by analysis of polymorphic STR markers, including the PCR technique and capillary electrophoresis. The method uses multiplex QF-PCR to multiply multiple fluorescently labelled DNA fragments of specific STR markers on the chromosomes tested in a single reaction. After amplification, DNA fragments are then separated, detected and analysed using capillary electrophoresis and appropriate software on a genetic analyzer. Fragments of individual STR markers are specified by the length and type of fluorescence labelling. Each fluorescently labelled DNA fragment appears as a peak with a certain height/area that is proportional to the amount of DNA. For testing, we use a standard set of markers (13, 18, 21, X and Y). The STR profile from maternal blood is determined and compared with the STR profile obtained from the amniotic fluid. In the case of a match, contamination is confirmed, and vice versa, when two different but related profiles are obtained, contamination is excluded.

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