PGT-M direct embryo sequencing
Test covered by the reimbursement:
YES
Clinical expertise code:
208 Test without reimbursement:
YES
Gender:
Woman/Man
Material:
Trophectoderm, Amplified DNA from the trophectoderm
Turnover time:
4 weeks
STATIM:
2 weeks
Material:
Trophectoderm
| Number of biopsies x 0.2 ml PCR tube with trophectoderm cells in laboratory-supplied buffer
Storage after examination: for 5 years after the report is issued -25°C – -15°C
Amplified DNA (WGA) from the trophectoderm
| 1x 0.2 ml PCR tube with aliquot of WGA product
Storage after examination: for 5 years after the report is issued -25°C – -15°C
Quick test description:
Testing for the presence of a known mutation in an embryo originating from one parent using (Sanger) sequencing of a specific region. Can only be done in conjunction with the PGT-M karyomapping method.
Test details:
Sanger sequencing is used to detect a known familial mutation in embryos. The DNA sequence (nucleotide sequence) is determined by PCR with fluorescently labelled nucleotides. The reaction products are then analysed during capillary electrophoresis on a sequencer, where fluorescent signals are captured with the help of a laser detector and then converted into a nucleotide sequence by computer processing to determine the sequence of the DNA section to be examined.