Paternity/relatedness test

Test without reimbursement:
YES
Gender:
Woman/Man
Material:
Peripheral blood, Buccal swab …
Turnover time:
3 weeks

Material:

Peripheral blood | 1x 3 ml of whole blood in K3 EDTA tube
Storage after examination: week after the report is issued 2 – 8°C
Buccal swab | 2x swab stick for buccal swab collection
Storage after examination: week after the report is issued 2 – 8°C
Isolated DNA from blood | 10–100 ng/μL of isolated DNA from blood in a PCR tube of at least 15 μL.
Storage after examination: stored in a DNA archive without restriction 15°C
Isolated DNA from chorionic villi | 30–100 ng/μL of isolated DNA from chorionic villi in a microtube (Eppendorf type)
Storage after examination: stored in a DNA archive without restriction 15°C
Isolated DNA from amniotic fluid | 30–100 ng/μL of isolated DNA from amniocentesis in a microtube (Eppendorf type)
Storage after examination: stored in a DNA archive without restriction 15°C
Isolated DNA from cordocentesis | 30–100 ng/μL of isolated DNA from cordocentesis in a microtube (Eppendorf type)
Storage after examination: stored in a DNA archive without restriction 15°C
DNA isolated from the product of conception | 50–100 ng/μL in microtube (Eppendorf type)
Storage after examination: stored in a DNA archive without restriction 15°C
Cultured cells | 1.5 ml of cultured cells in a microtube (Eppendorf type)
Storage after examination: 180 days 2 – 8°C

Quick test description:

Determination of paternity or relatedness by analysis of 16 STR markers.

Test details:

Paternity and relatedness tests are performed by multiplex PCR followed by fragmentation analysis. 16 traits (15 STR markers + amelogenin marker to determine gender) are analysed. These traits create a characteristic genetic profile that is unique to each person except for identical twins. 

Paternity is determined by comparing the genetic profile of the child with that of the alleged father. If a combination of markers is found in a child that the alleged father does not have, paternity can be excluded with 100% certainty. 

If both profiles (of the child and the alleged father) show a match, paternity can be confirmed with certainty, which is close to 100%. The laboratory performs two types of tests: determination of paternity for private purposes for those clients who need to verify who the biological parent is, and forensic expert opinion, which is intended for legal proceedings (evidence in court, public prosecutor). For a forensic expert opinion, it is necessary to collect the sample in accordance with the protocol for the identification of persons (validity of identity documents, taking of a right thumb print in adults, photograph), which is done by a forensic expert in person. It is therefore necessary to make an appointment with them. If an expert opinion is not required, an analysis of the mother, father and child can be made, or only the father and child without examination of the mother. 

In this case, it is not necessary to carry out protocol collection in the presence of a forensic expert. However, the identity of the examined person is always verified according to the submitted documents (ID card, passport, birth certificate) – we perform these analyses only in exceptional cases with regards to the interests of the minor child and always with the consent of the child’s legal guardian.

Indication

the testing is carried out on the basis of an order of applicants for testing (private persons, courts, state prosecutors, or other law enforcement authorities) – self-payers; the testing is not covered by public health insurance

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