Detection of aberrations by FISH
Test covered by the reimbursement:
YES
Gender:
Woman
Material:
Chorionic villi, Amniotic fluid
…
Turnover time:
4 weeks
STATIM:
1 week
Material:
Chorionic villi
| Chorionic villi, min. 30 mg tissue in a microtube (Eppendorf type)
Storage after examination: week after the report is issued 2 – 8°C
Amniotic fluid
| 3x 10 ml of amniotic fluid in a tube
Storage after examination: week after the report is issued 2 – 8°C
Cord blood
| 2–3 ml of cord blood in EDTA
Storage after examination: week after the report is issued 2 – 8°C
Cultured cells
| 1.5 ml of cultured cells in a microtube (Eppendorf type)
Storage after examination: 180 days 2 – 8°C
Quick test description:
Genetic testing to specify structural chromosomal aberrations or to accurately quantify mosaicism in a sample.
Test details:
The FISH (Fluorescence In-Situ Hybridization) method uses the principle of double-stranded DNA hybridization and fluorescence microscopy. The DNA probe is prepared from a specific region of the human genome and is labelled with a covalently bound fluorescent dye. Different fluorescent dyes differ in excitation and emission wavelengths. By scanning the cell nuclei in different fluorescent channels, the number of fluorescent signals for individual chromosomes or their parts in individual cells and overall in the sample is evaluated.